深藍色寄居蟹的窩

http://www.youtube.com/watch?v=x5yPkxCLads 

The PCR Song

There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.

Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.

Just mix your template with a buffer and some primers,
Nucleotides and polymerases, too.

Denaturing, annealing, and extending.
Well it's amazing what heating and cooling and heating will do.

PCR, when you need to detect mutations.
PCR, when you need to recombine.
PCR, when you need to find out who the daddy is.
PCR, when you need to solve a crime.

曾經有段日子，每當想要擴增 DNA的時候
人們就必須養一大堆一大堆的小小細胞
然後出了個叫 Kary Mullis博士的傢伙
他說其實在生物體外的試管中就可以做啦
只要把 DNA模板跟緩衝液與引子混合起來
再加上核甘酸跟聚合酵素
變性、黏合、延長 ──
加熱降溫再加熱就能搞定的事還真是神奇！
當你需要偵測突變，跑個PCR 吧!
當你想要重組DNA ，跑個PCR 吧!
當你想知道到底誰才是親生的阿爸，跑個PCR 吧!
當你想要破解罪案的時候，跑個PCR 吧!